Polo is an Italian and Spanish surname, the most well known bearing the name being Italian trader and explorer Marco Polo (1254–1324). Other notable people with the surname include:
Niccolò and Maffeo Polo (1230–1294 and 1230–1309 respectively), Marco Polo's father and uncle respectively
Aldo Polo (born 1983), Mexican footballer
Ana María Polo (born 1959), Cuban-American lawyer and Hispanic television arbitrator
Andy Polo (born 1994), Peruvian footballer
Armando Polo (born 1990), Panamanian footballer
Asier Polo, Spanish cellist
Bernardo Polo (died c.